Biology

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emeraldtermite679Lv1

28 Sep 2019

you incubate tubes for 24 hours. In both the PRB glucose tubeand PRB lactose tube you see yellow broth and an air bubble in thedurham tube. What does this means?

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Reid WolffLv2

28 Sep 2019

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Related questions

Question 1

What is the last step of successfully preparing a bacterial smear for a simple stain?

		Spread cells with inoculating loop
		Label the slides
		Heat fixation
		Air dry the smear

Question 2

When looking at your smear under the microscope, all you see is darkness (despite shining light through your slide)- the best explanation is:

		all your bacteria are dead
		your smear was too thin
		forgot to add the bacteria
		your smear was too thick

Question 3

If a microbe can ferment lactose and its fermentation products include lactic acid and CO2, then the results for this assay should be:

		the broth will appear yellow and there will be a gas bubble in the Durham tube
		the broth will appear red and there will be no gas bubble in the Durham tube
		the broth will appear yellow and there will be no gas bubble in the Durham tube
		the broth will appear red and there will be a gas bubble in the Durham tube

True/Flase

Question 1

If the result for this carbohydrate test is negative, then the organism had NO growth in the tube.

Question 2

The biochemical basis of the Gram stain is the thickness of peptidoglycan layers surrounding cells. Therefore a Gram positive cell with a thin layer of peptidoglycan should stain as Gram negative.

Question 3

If the iodine is not applied during the Gram stain, then gram positive cells will likely stain pink

Question 4

A simple stain allows you to see the cell shape and arrangement.

Question 5

If there were no limit to magnification, a light microscope could enlarge microbes infinitely.

Question 6

When doing a bacteria smear from a broth medium you should first place one to two loopfuls of water on the center of the slide?

2. Please answer with a True or False for the following:

a. The catalase enzyme is found in all obligate aerobes and facultatuve anaerobes to deal eith H2O2

b. An exergonic reaction requires energy input and must be coupledwith ATP hydrolysis in order to proceed

c. The TCA cycle must "turn twice" in order to complete the oxidation of one glucose molecule.

d. Allosteric regulation occurs when an enzyme has a site that can bind an effector molecule seperate from the active site.

e. Some bacteria use desulfurases to produce hydrogen sulfide from he breakdown of some amino acids.

f. A 1/10 dilution can be made by adding 1 mL sample to 10mL diluent

3. You obtain the following results after incubation of a bacterial starin in tge indicated carbohydrate fermemtation sugar media:

Sugar: Color of Medium Durham tube results

Glucose Yellow No bubble

Maltose Red Bubble

Mannitol Yellow No bubble

Lactose Yellow Bubble

Sucrose Red No Bubble

Which sugars cannot be fermented by this bacterial strain?

a. glucose and mannitol

b. maltose and lactose

c. lactose

d. maltose and sucrose

e. none of the choices are correct

raspberrykoala332

1. You smear a small a mount of culture onto a strip containing a reagebt that tests for the presence of oxidase. The inoculated spot turns dark blue within 20 seconds. What does i mean?

a. The culture deos not contain oxidase

b. The culture is likely an aerobe

c. The culture is likely an anaerobe

d. The culture does not contain catalase

E. None of the above

2. True or False