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What is the main conceptual difference between the Maxam-Gilbert sequencing method and the Sanger sequencing method?

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Bunny Greenfelder
Bunny GreenfelderLv2
28 Sep 2019

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Related questions

PLEASE ANSWER ALL PARTS FULLY!

How was liquid hybridization used to compare species relatedness? What is the purpose/significance of Driver versus Tracer DNA? What is Subtractive Hybridization, how/why would you use this technique?

Compare Maxam-Gilbert and Sanger DNA sequencing methods. What are the more descriptive names for these techniques? How are the techniques similar? different? What reagents are used in each reaction? How are the results read? Do you get the sequence of the molecule you started with or the complementary molecule? How does fluorescent cycle sequencing differ from the original Sanger Method?

For reading sequences in Maxam Gilbert sequencing - I understandthat you go from top to bottom, but what if there is one colored infragment at G and one blank fragment at A on the same level - whatdoes this mean? Thank you.

DNA Sequencing (Sanger method):

https://www.dnalc.org/resources/animations/sangerseq.html

Questions:

5. In what ways are PCR and Sanger sequencing similar?

6. What is the important difference in Sanger sequencing?

7. Why is size separation of DNA fragments so important in Sanger sequencing? How are the DNA fragments separated in the animation?

Additional videos on Sanger DNA sequencing:

Sanger Sequencing

https://www.youtube.com/watch?v=6ldtdWjDwes

The Sanger Method of DNA Sequencing

https://www.youtube.com/watch?v=FvHRio1yyhQ

____________________________________________________________________________

Bacterial Transformation:

https://www.dnalc.org/resources/animations/transformation1.html

Questions:

8. What are plasmids?

9. What was used to cut the plasmids?

10. What was used to seal the DNA fragments together?

11. How did the researchers select for bacteria that contain both antibiotic resistance genes?

12. What is a recombinant plasmid?

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