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Determine which is the result of complementation or which fails to complement in terms of gene interaction. The two options for answers are as follows: a) complementation or b) not complement Please spell the answers and report in all lowercase correctly to receive full points. Thus, the answer to the question shall either be "complementation" or "not complement".

Questions for determination:

1) Wild type progeny Blank 1

2) Not wild type progeny Blank 2

3) Null mutation Blank 3

4) Recessive mutation Blank 4

5) Alleles of the same gene Blank 5

6) Alleles of different genes Blank 6

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Trinidad Tremblay
Trinidad TremblayLv2
28 Sep 2019
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Related questions

1. Haploid Saccharomyces cerevisiae yeast cells contain the following alleles at the MAT locus.

a. Allele a

b. Allele alpha

c. Allele a or alpha

d. Alleles a and alpha

2. The following statements are true for the Saccharomyces cerevisiae yeast, except __________.

a. Yeast mating type a produces a-factor.

b. Yeast mating type alpha produces alpha-factor.

c. a-factor or alpha-factor signals the presence of a yeast cell to the neighboring cells.

d. Yeast mating type a-cell responds to a-factor, alpha-cell responds to alpha-factor.

3. A yeast strain can sexually conjugate with another yeast strain of the same mating type.

a. True

b. False

4.Heterothallic Saccharomyces cerevisiae yeast strains commonly used in controlled experiments are haploid, with either mating type a or alpha.

a. True

b. False

5. Complementation test is used to ____________________________.

a. determine the mating type of yeast.

b. determine whether mutations occur at the same gene or different genes.

c. identify mutant organisms.

d. determine functional relationships between genes.

6. Yeast cells with mating type alpha can sexually conjugate with yeast _________.

a. mating type a

b. mating type alpha

c. allele a or alpha

d. alleles a and alpha

7. Complementation test is conducted by ___________ two strains of organisms with different mutations.

a. isolating

b. morphologically identifying

c. growing

d. intercrossing

8. Assuming alleles B or b are found in locus 1; alleles C or c are found in locus 2. Which of the yeast strains below contains recessive mutations at loci 1 and 2?

a. Yeast strain with alleles B and C

b. Yeast strain with alleles B and b

c. Yeast strain with alleles C and c

d. Yeast strain with alleles b and c

9. When mutations occur at different genes, two strains of yeast are considered to be not complement.

a. True

b. False

10. Yeast cells that are ready to mate will produce a mating projecting called _______ to form a pear-shape cell.

a. pilus

b. yeast ring

c. shmoo

d. spore

You have four pure-breeding strains of mice that all have white fur. Crosses between brown females from a wild-type strain and white males from your four strains produce only brown progeny, indicating that all four strains carry a recessive mutation that causes white fur. You cross all of the white strains to each other as indicated in the table below, and record the fur color of the offspring.

Mother strain Father strain Progeny

Cross #1 A B brown

Cross #2 A C brown

Cross #3 A D white

Cross #4 C B white

Cross #5 D B brown

Cross #6 D C brown

Cross #7 B A brown females, white males

Cross #8 C A brown females, white males

Cross #9 D A white

Cross #10 B C white

Cross #11 B D brown females, white males

Cross #12 C D brown females, white males

Complete the following table of progeny genotypes from the crosses listed above. You will need as many loci (columns) as there are number of different genes carrying white-fur mutations (this may be more or fewer than three loci). In each row indicate the genotype of the progeny of that cross at all loci. Denote white-fur alleles with w and brown-fur alleles with +. If there are loci where the genotype differs between males and females, list both genotypes and indicate which genotype belongs to each sex.

Cross # locus1 locus2 locus3 ....

1 + +

2

3

4

5

6

7

8

9

10

11

12

In a few sentences, describe why some crosses produce all brown offspring, some crosses produce all white offspring, and some produce brown and white offspring. Use the following terms in your answer: complement, fail to complement, X-linked, autosomal.

You have done a genetic screen to identify genes controlling wings in an insect. Some of the mutants have no wings, some have short wings and others have larger than normal wings.

1a. (9 points) What type of mutation (e.g., amorph, hyopmorph, etc.) is most likely responsible for each class of phenotypes? Explain briefly. State whether you expect each to be recessive or dominant.

No wings:

Short wings:

Large wings:

Mutation

1

2

3

4

5

6

7

8

9

10

11

12

1

-

+

+

+

-

+

+

+

+

+

+

+

2

-

+

+

+

-

+

-

+

-

+

+

3

-

-

+

+

+

+

+

+

+

+

4

-

+

+

+

+

+

+

+

+

5

-

+

+

+

+

+

+

+

6

-

+

-

+

-

+

+

7

-

+

+

+

-

-

8

-

+

-

+

+

9

-

+

+

+

10

-

+

+

11

-

-

12

-

1b. (2 points) How many genes causing the small wing phenotypes are revealed by this analysis? ___________

1c. (6 points) Which mutants belong to the same complementation groups?

1d. (3 points) Cloning of the gene causing the large wings phenotype reveals that is a growth regulator, which is a ubiquitous protein present in many different cell types. Suggest an hypothesis to explain why only the wings were large but not other tissues.

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