Question

For each of the following engineered proteins, predict which location in the cell they would be targeted to. Assume the following:

- Each targeting sequence is at the site along the protein (N terminus, C terminus, internal) where it would normally be found.

- Only targeting sequences possessed by each protein are those listed.

a) A cleavable ER-signal sequence and a stop transfer sequence (answer is not ER)

b) A peroxisomal sequence and a lysosomal signal sequence (answer is not lysosome)

c) No sorting signal (answer is not ER)

d) Multiple start and stop sequence signals (answer is not membrane of ER)