A biochemist was studying the structure of a normal (native) and mutant form of a globular protein. Both proteins could be crystallized, but the mutant protein had a different tertiary structure than the native protein.
1. The biochemist determined that an important structural region of the native protein was encoded for by the following mRNA sequence:
(5â) GCCAAAAGUACAGCAUGGCAA (3â)
Using the table of the genetic code (lecture 12, slide 10), list from the N-terminus to C-terminus the corresponding amino acid sequence for the native protein:
The same region of the mutated protein was encoded by the following mRNA sequence:
(5â) GCCAACAGUACAGCAGGGCAA (3â)
List from the N-terminus to C-terminus the corresponding amino acid sequence for the mutant protein:
2. Propose a plausible explanation for why the mutations led to a change in the tertiary structure of the native protein.
A biochemist was studying the structure of a normal (native) and mutant form of a globular protein. Both proteins could be crystallized, but the mutant protein had a different tertiary structure than the native protein.
1. The biochemist determined that an important structural region of the native protein was encoded for by the following mRNA sequence:
(5â) GCCAAAAGUACAGCAUGGCAA (3â)
Using the table of the genetic code (lecture 12, slide 10), list from the N-terminus to C-terminus the corresponding amino acid sequence for the native protein:
The same region of the mutated protein was encoded by the following mRNA sequence:
(5â) GCCAACAGUACAGCAGGGCAA (3â)
List from the N-terminus to C-terminus the corresponding amino acid sequence for the mutant protein:
2. Propose a plausible explanation for why the mutations led to a change in the tertiary structure of the native protein.
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