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Complete the following to describe the role that an operon plays in the synthesis of enzymes in prokaryotes.

An operon consists of an operator gene, a promoter gene, and the structural genes. When a repressor is bound to the operator, RNA polymerase cannot bind to the _________operatorpromoterstructural genes to start transcription of the _________operatorpromoterstructural genes. When a(n) _________helicaseinducerrepressorribosome is present, it binds to and inactivates the _________helicaseinducerrepressorribosomeRNA polymerase. This inactive _________helicaseinducerrepressorribosomeRNA polymerase can no longer bind to the operator. As a result, _________helicaseinducerrepressorribosomeRNA polymerase can bind to the promoter, leading to eventual transcription of the _________operatorpromoterstructural genes that encode the enzymes to be synthesized.

-operator

-promoter

-structural genes

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Jarrod Robel
Jarrod RobelLv2
28 Sep 2019
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Related questions

Fill in the blank:

promotor, induction, presence, will not, will, operon, repressor, repression, overabundance, operator, control region,

1. If the repressor is bound to the operator, then RNA polymerase______ transcribe the structural genes.

2. The ________ controls whether RNA polymerase can advance along the DNA strand or not.

3. The _______ operates differently depending on whether the operon is inducible or repressible.

4. An _________ controls the expression of functionally related genes and consists of a control region and structural genes.

5. ______ decreases expression of the structural genes. This typically occurs in response to an _____ of a substrate.

6. The _____ contains a promoter and an operator.

7. If the repressor is not bound to the operator, then RNA polymerase _____ transcribe the structural genes.

8. ______ increases expression of the structural genes. This typically occurs in response to the _______ of a substrate.

9. The ______ is the region of DNA where RNA polymerase initially binds.

In the following points, I have summarized Lac operon in Ecoli. I need to check if this is on the right track? and where does 'cleavage of disaccharides' fit into this picture?

Lactose is a disaccharide and the bacteria need specific genes to manage catabolism

1) The lacl promotor gene synthesis the repressor protein which binds to the operator gene stopping the RNA polymerase from coding the lac operon genes

2)But when lactose is present, it needs the specific genes to catabolise lactose

3) So, When allolactose (a derivative of lactose)is present, it binds to the repressor protein, the repressor releases from the operator gene

4) This allows the RNA polymerase can attach to the DNA control region

5) And drive down the operon for transcription and induction of the enzyme lacZ(beta-galactosidase) lacY and lacA which breaks lactose down into glucose and galactose

6) When the lactose is all gone, the repressor returns to the operator in a feedback loop system

A theoretical operon (amo) in E. coli contains several structural genes encoding enzymes involved in the biosynthesis of a particular amino acid. Unlike the lac operon, in which the repressor gene is separate from the operon itself, for the amo operon, the repressor gene is found within the operon itself. When the end product amino acid is present, it binds to the repressor, and this complex binds to the operator, repressing the operon. In the absence of the amino acid, the repressor fails to bind to the operator, and transcription proceeds. Characterize this operon, comparing it to the regulation of the lac and trp operons. Be sure to discuss how regulation is affected by having the repressor gene as part of the operon.

Then, consider the following mutations:

a) Mutation in the operator region;

b) Mutation in the promoter region;

c) Mutation in the repressor gene.

In each case, state whether the operon will be transcribed or not, and describe how the mutation affects the regulation of the operon. Compare each response to the equivalent situation in the lac and trp operons.

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