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Which prokaryotic mRNA sequence helps position the ribosome for initiation of translation?

A. Shine-Dalgarno sequence

B. TATA box

C. Okazaki sequence

D. PolyA tail

E. Kozak sequence

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Beverley Smith
Beverley SmithLv2
28 Sep 2019
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Related questions

1. What is the Shine-Dalgarno sequence? What binds to the sequence during translation? Describe some of the ways in which the sequence can vary, addressing the consequences that any such variation has on translation efficiency. In Detail

2. Describe the A, P, and E sites of the ribosome. Where are they located? What occupies each of the sites during the following stages of translation:

a. upon creation of the 70s initiation complex

b. after an initial round of peptidyl transfer, prior to aminoacyl tRNA binding

c. following aminoacyl tRNA binding, but before the peptidyl transfer reaction

d. after peptidyl transfer, but before translocation is completed by EF-G

e. in the presence of puromycin, prior to peptidyl transfer

f. upon reaching a stop codon

g. following release of the polypeptide chain, but before the appearance of RRF

3’ UTR

5’ UTR

promoter

enhancer

operator

intron

transcription termination site

polyadenylation signal/site

Shine-Delgarno sequence

5’ cap

3’ cleavage site

consensus sequence

a. For the gene or mRNA components listed above, write next to each one whether it typically occurs in prokaryotic genes/mRNA only (P), eukaryotic genes/mRNA only (E), or both (B).

b. List the known functions of the 5’ cap and the poly A tail.

Here you have the non-template strand of DNA sequences of genomic DNA and mRNA of the human insulin gene (1431nt long from +1nt).

……….CAAT…..<60nt>…..TATA..<28nt>..AGCCCTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGGTCTGTTCCAAGGGCCTTTGCGTCAGGTGGGCTCAGGATTCCAGGGTGGCTGGACCCCAGGCCCCAGCTCTGCAGCAGGGAGGACGTGGCTGGGCTCGTGAAGCATGTGGGGGTGAGCCCAGGGGCCCCAAGGCAGGGCACCTGGCCTTCAGCCTGCCTCAGCCCTGCCTGTCTCCCAGATCACTGTCCTTCTGCCATGGCCCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGCTGGCCCTCTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGGTGAGCCAACTGCCCATTGCTGCCCCTGGCCGCCCCCAGCCACCCCCTGCTCCTGGCGCTCCCACCCAGCATGGGCAGAAGGGGGCAGGAGGCTGCCACCCAGCAGGGGGTCAGGTGCACTTTTTTAAAAAGAAGTTCTCTTGGTCACGTCCTAAAAGTGACCAGCTCCCTGTGGCCCAGTCAGAATCTCAGCCTGAGGACGGTGTTGGCTTCGGCAGCCCCGAGATACATCAGAGGGTGGGCACGCTCCTCCCTCCACTCGCCCCTCAAACAAATGCCCCGCAGCCCATTTCTCCACCCTCATTTGATGACCGCAGATTCAAGTGTTTTGTTAAGTAAAGTCCTGGGTGACCTGGGGTCACAGGGTGCCCCACGCTGCCTGCCTCTGGGCGAACACCCCATCACGCCCGGAGGAGGGCGTGGCTGCCTGCCTGAGTGGGCCAGACCCCTGTCGCCAGGCCTCACGGCAGCTCCATAGTCAGGAGATGGGGAAGATGCTGGGGACAGGCCCTGGGGAGAAGTACTGGGATCACCTGTTCAGGCTCCCACTGTGACGCTGCCCCGGGGCGGGGGAAGGAGGTGGGACATGTGGGCGTTGGGGCCTGTAGGTCCACACCCAGTGTGGGTGACCCTCCCTCTAACCTGGGTCCAGCCCGGCTGGAGATGGGTGGGAGTGCGACCTAGGGCTGGCGGGCAGGCGGGCACTGTGTCTCCCTGACTGTGTCCTCCTGTGTCCCTCTGCCTCGCCGCTGTTCCGGAACCTGCTCTGCGCGGCACGTCCTGGCAGTGGGGCAGGTGGAGCTGGGCGGGGGCCCTGGTGCAGGCAGCCTGCAGCCCTTGGCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTACTGCAACTAGACGCAGCCCGCAGGCAGCCCCACACCCGCCGCCTCCTGCACCGAGAGAGATGGAATAAAGCCCTTGAACCAGC

The mRNA strand is as follows:

mRNA sequence (465nt long)

AGCCCUCCAGGACAGGCUGCAUCAGAAGAGGCCAUCAAGCAGAUCACUGUCCUUCUGCCAUGGCCCUGUGGAUGCGCCU CCUGCCCCUGCUGGCGCUGCUGGCCCUCUGGGGACCUGACCCAGCCGCAGCCUUUGUGAACCAACACCUGUGCGGCUCA CACCUGGUGGAAGCUCUCUACCUAGUGUGCGGGGAACGAGGCUUCUUCUACACACCCAAGACCCGCCGGGAGGCAGAGG ACCUGCAGGUGGGGCAGGUGGAGCUGGGCGGGGGCCCUGGUGCAGGCAGCCUGCAGCCCUUGGCCCUGGAGGGGUCCC UGCAGAAGCGUGGCAUUGUGGAACAAUGCUGUACCAGCAUCUGCUCCCUCUACCAGCUGGAGAACUACUGCAACUAGA CGCAGCCCGCAGGCAGCCCCACACCCGCCGCCUCCUGCACCGAGAGAGAUGGAAUAAAGCCCUUGAACCAGC

QUESTIONS:

1) Label the following on the DNA strand:

a) +1 position

b) upstream and downstream sequence relative to +1 nucleotide

c) Promoter region

d) Exons

e) introns

f) splicing signals (dinucleotides)

2. Label the following on the mRNA strand:

a) Start codon

b) stop codons

C) Kozak sequence

3. If you placed this gene (human insulin) in a bacterial cell, do you think a functional insulin product will be made? Why or why not?

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