DNA ISOLATION/GEL ELECTROPHORESIS HW
1. During agarose gel electrophoresis, the cause of drag on a linear DNA molecule is the:
a. Interaction with TAE buffer
b. Nitrogen bases in the DNA molecule
c. Interaction with Ethidium Bromide in the gel
d. Agarose in the gel
2. As part of your prelab work, you should have looked at the DNA isolation protocol. Why do you boil your tubes?
a. To disrupt the cell and nuclear envelope to release the DNA
b. To seperate the DNA from other cellular debris
c. To denature the DNA for PCR, the next step
d. To isolate cheek cells3.
3. At the end of your DNA isolation procedure you will have Chelex beads and some liquid. Explain which layer has your DNA (and thus you will save) and why.
4. What is the electric charge (q) on a 200bp linear piece of DNA?
DNA ISOLATION/GEL ELECTROPHORESIS HW
1. During agarose gel electrophoresis, the cause of drag on a linear DNA molecule is the:
a. Interaction with TAE buffer
b. Nitrogen bases in the DNA molecule
c. Interaction with Ethidium Bromide in the gel
d. Agarose in the gel
2. As part of your prelab work, you should have looked at the DNA isolation protocol. Why do you boil your tubes?
a. To disrupt the cell and nuclear envelope to release the DNA
b. To seperate the DNA from other cellular debris
c. To denature the DNA for PCR, the next step
d. To isolate cheek cells3.
3. At the end of your DNA isolation procedure you will have Chelex beads and some liquid. Explain which layer has your DNA (and thus you will save) and why.
4. What is the electric charge (q) on a 200bp linear piece of DNA?
