Question

1. During agarose gel electrophoresis, the cause of drag on a linear DNA molecule is the:

a. Interaction with TAE buffer

b. Nitrogen bases in the DNA molecule

c. Interaction with Ethidium Bromide in the gel

d. Agarose in the gel

2. As part of your prelab work, you should have looked at the DNA isolation protocol. Why do you boil your tubes?

a. To disrupt the cell and nuclear envelope to release the DNA

b. To seperate the DNA from other cellular debris

c. To denature the DNA for PCR, the next step

d. To isolate cheek cells