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silvershark268Lv1

28 Sep 2019

When a normal lung cells becomes a lung cancer cell, there are several important changes in plasma membrane properties. How would you investigate the following phenomena? The cancer cell membrane is more fluid with more movement of both lipids and proteins The cancer cell has altered adhesion properties, binding to other tissues in addition to lung cells

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Reid WolffLv2

28 Sep 2019

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For each of the following, indicate what experimental approach you would use to answer the given scenarios a-e. You must include the following:

1. Experimental approach

2. General outline of procedure for that approach. That is, an overview of how the protocol is conducted, including critical reagents or steps.

3. Expected results, including any specific marker proteins associated with the assay that would change or vary.

Here is an example:

Protein Z is important in the migration of prostate cancer cells.

1. Migration scratch assay

2. Transfect siRNA for Protein Z and control siRNA into prostate cancer cells. Grow to confluence, generate scratch, photograph scratches at different time points, measure movement of cells into scratch. Determine relative rate of movement of cells over time.

3. Result: Knockdown of Protein Z reduces the rate of movement of cells

a. Protein X can cause cellular transformation when expressed in a normal lung cell line.

b. Tumor cells cause changes in cell surface marker protein B of associated immune cells (TAMâs or tumor associated macrophages).

c. Transcription Factor Y is critical for the EMT phenotype of breast cancer during progression.

d. High expression of Protein Q decreases patient survival.

e. A CAF (cancer associated fibroblast) can cause greater migration of breast tumor cells.

cyancricket98

For each of the following, indicate what experimental approach you would use to answer the given scenarios (a-e). You must include the following:

1. Experimental approach

2. General outline of procedure for that approach. That is, an overview of how the protocol is conducted, including critical reagents or steps.

3. Expected results, including any specific marker proteins associated with the assay that would change or vary.

Here is an example:

Protein Z is important in the migration of prostate cancer cells.

1. Migration scratch assay

3. Result: Knockdown of Protein Z reduces the rate of movement of cells

a. Protein X can cause cellular transformation when expressed in a normal lung cell line.

b. Tumor cells cause changes in cell surface marker protein B of associated immune cells (TAMâs or tumor associated macrophages).

c. Transcription Factor Y is critical for the EMT phenotype of breast cancer during progression.

d. High expression of Protein Q decreases patient survival.

e. A CAF (cancer associated fibroblast) can cause greater migration of breast tumor cells.

blushsardine369

1. How does the extracellular matrix affect a cell that it surrounds? (Select all that apply.)

	The cell's shape depends in part on the structure and composition of the extracellular matrix.
	The genes that the cell expresses depend in part on the types of proteins in the extracellular matrix.
	The size that the cell grows to depends in part on the polysaccharides in the extracellular matrix.
	The extracellular matrix has no effect on the cells that it surrounds.
	All of these choices are correct.

2. Which of the following enable(s) the movement of cells?

	microtubules
	microfilaments
	intermediate filaments
	microtubules and microfilaments
	microfilaments and intermediate filaments

3. Microtubules increase in length:

	more quickly at one end than the other.
	by growing outward from the centrosome.
	in cycles, following rapid depolymerization.
	if free tubulin dimers are available.
	All of these choices are correct.

4. Dynamic instability is the rapid assembly and disassembly of:

	microfilaments.
	intermediate filaments.
	microtubules.
	both microfilaments and microtubules.

5. Which one of the following is a characteristic shared by integrins and cadherins?

	Both are peripheral membrane proteins.
	Both facilitate the adhesion of cells to extracellular matrix proteins.
	Both are present in adherens junctions, desmosomes, and hemidesmosomes.
	The cytoplasmic domains of both proteins are connected to the cytoskeleton.