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What is the total magnification at low power of this microscope? (ocular lens: 10x, scanning power: 4x, low power: 10x, high power: 40x, oil immersion: 100x)

If a colorless substance has a negative Benedict’s test, what color is the test tube?

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Bunny Greenfelder
Bunny GreenfelderLv2
28 Sep 2019

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Related questions

LAB MODULE: BASIC MICROSCOPY – STUDY HELP

The information in this document is helpful in preparing for the Basic Microscopy Pre-Lab Quiz on Canvas and the Basic Microscopy quiz on Late Nite Labs.

Instructions:

• Go to your Late Nite Labs course site and click on the Basic Microscopy link. The lab manual content will appear on the right side of the screen.

• PRINT and READ all of the information found in the Basic Microscopy lab manual.

• Use the information to answer the following questions.

Questions

• Fill in the remainder of the chart.

Objective Lens

Objective Magnification

Ocular Magnification

Total Magnification

Scanning

4X

10X

40X

Low Power

10X

10X

High Power

40X

10X

Oil Immersion

100X

10X

• Do all organisms consist of more than one cell?

• What is the structural and functional unit of living things? In other words, what is considered the basic building block of LIFE?

• What three structures are found in the cells of ALL organisms, both prokaryotic and eukaryotic? You may have to look at the pictures of the cells in the Basic Microscopy lab manual content to find all three structures. Note: plasma membrane = cell membrane

• List the major differences in prokaryotic cells and eukaryotic cells? Include the location of genetic material in a eukaryotic versus prokaryotic cell.

• List the organelles found in a eukaryotic cell that are not found in a prokaryotic cell. You may have to look at the pictures of the cells in the Basic Microscopy lab manual content to find all of these.

• In eukaryotic cells, genetic information is found in the form of DNA. Is this true for prokaryotic cells?

• Are flagella and cell walls unique to eukaryotes? Why or why not?

• The __________is the part of a cell that allows the movement of substances into and out of the cell and protects the cell from its environment.

• The__________is a substance that contains all of the dissolved and suspended ions and other molecules needed to keep the cell alive.

• A__________is an organelle that serves as the site of photosynthesis in a eukaryotic cell.

• Some organisms have cells that contain a__________that gives the cell a more structured shape and strengthens the cell by osmotic pressure.

• Describe dark field microscopy.

• Describe phase contrast microscopy.

• Why do scientists use dark field microscopy and phase contrast microscopy when studying prokaryotic cells?

• How does phase contrast microscopy help scientists visualize difficult specimens?

• Fill in the remainder of the chart. The organisms studied during this lab are: Amoeba, Spirogyra, cardiac muscle, and bacteria.

Structure Function Organisms Studied
Plasma Membrane

Amoeba, Spirogyra, cardiac muscle, and bacteria
Cytoplasm
Nucleus

Contains the genetic material of a eukaryotic cell
Chloroplast

Spirogyra
Cell wall

Circle the correct answer from choices shown in bold or fill inthe blank:

1-When necessary, adjust the intensity of the slide’sillumination using the light source / condenser.The contrast of the image can be adjusted using theiris diaphragm / condenser.Contrast is (briefly explain in one sentence):__________________________________________________________________.

Loss of contrast usually results from too little / toomuch illumination.

2. Never use the 4X / 10X / 40X / 100Xobjective without immersion oil. In this course, will you be usingthis objective? Yes / No.

3- Before removing a slide from the stage, lower /raise the stage and return to the 4X / 10X / 40X /100X objective. When completely finished using themicroscope, leave the light source on / turn off the lightsource.

4. Give the numerical aperture values for the followingobjectives (get these values from the microscope in front of you):4X _______, 10 X _______ and 40 X _______. Numerical aperture meanswhat? __________________________________________.

5-The ability to perceive or distinguish two adjacent points asseparate entities on an image is called ____________. It used as anindicator of lens / specimen quality. An increasein magnification can lead to a reduction in ____________ and____________.

5. What does the term parfocal mean in microscopy? Give a briefdescription.______________________________________________________________________

1. Explain how total magnification is calculated. Provide an example

2. What effect does increased magnification have on the field of view?

Remember, field of view refers to the total amount of the object that you can see.

3. Why should you always begin focusing on a specimen with the 4X objective lens in place?

4. What is the proper way to carry a microscope?

5. What is the proper method for cleaning the microscope?

6. What is the purpose of the iris diaphragm?

7. Why is it important to have an understanding of the concept of depth of field?

8. Why must immersion oil be used when viewing a specimen under the 100X objective lens?

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