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Aseptic Technique and Bacterial Anatomy and Morphology Labreport.

The first experiment Aseptic Technique.

The second experiment is Bacterial Anatomy and Morphology Labreport

In this experiment, you will view the Gram Stain results for twodifferent samples. The purpose of this experiment is for you togain an understanding of how the morphology and arrangement ofbacteria relate to staining techniques.

Introduction?

Purpose Findings?

Procedure?

Observations and Results?

Discussion?

Conclusion?

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Reid Wolff
Reid WolffLv2
28 Sep 2019
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Related questions

ASEPTIC TECHNIQUE

Introduction Procedure < Include information that the readerwould need to repeat your experimental procedure. Observations andResults Gram stain result: (Experiment 1 and 2) Part I:Contamination Step 6: What color are the traffic lights after capsare removed? Step 7: What color are the traffic lights after capshave been left off and then replaced? Step 9: Appearance of tube:Step 12: Appearance of tube after overnight incubation: Part II:Aseptic Transfer Tube-to-Tube Test Was your aseptic transfersuccessful? (Contamination light and Inoculation light both green)Appearance of tube after overnight incubation: Discussion In theDiscussion section, answer the following questions: Whatprecautions are taken in a "wetlab" to prevent contamination?During the "Contamination" portion of the exercise, why was thecontamination not present when you first placed the phenol redglucose tube in the 37 degree incubator? After your sample had beenincubated for one day, why is the solution turbid? What did thecolor change of the medium indicate? What caused the contaminationof your sample? How successful were you with the aseptic technique?How many times did you need to repeat the experiment until you wereable to successfully inoculate your media without contamination?How important is sterile technique in a "wetlab?" Why? Conclusion< Within a few sentences, provide a concluding statement aboutthe results of your laboratory>

BACTERIAL ANATOMY Introduction Procedure < Includeinformation that the reader would need to repeat your experimentalprocedure. Do not include any observations or results in thissection> Observations and Results Gram stain result: (Experiment1 and 2) Discussion In the Discussion section, answer the followingquestions: Describe the morphology of each bacterium, e.g., cocci,rods, or spiral/curved. Describe the arrangement of the bacteria.Were the bacteria organized as singles, pairs, chains, clusters, oranother type of arrangement? Describe the Gram Stain procedure andhow to interpret the results. How does this apply to your Sample #1and Sample #2? Which stain is responsible for the results that yousee? Relate the results to cell structure. You can also useInternet resources. Make sure that you cite all external datasources, including your textbook. Indicate the terminology that isused for the cell anatomy and arrangement observed for the organismin Sample #1 and Sample #2.

ASEPTIC TECHNIQUE Introduction Procedure < Includeinformation that the reader would need to repeat your experimentalprocedure. Do not include any observations or results in thissection> Observations and Results Gram stain result: (Experiment1 and 2) Part I: Contamination Step 6: What color are the trafficlights after caps are removed? Step 7: What color are the trafficlights after caps have been left off and then replaced? Step 9:Appearance of tube: Step 12: Appearance of tube after overnightincubation: Part II: Aseptic Transfer Tube-to-Tube Test Was youraseptic transfer successful? (Contamination light and Inoculationlight both green) Appearance of tube after overnight incubation:Discussion In the Discussion section, answer the followingquestions: What precautions are taken in a "wetlab" to preventcontamination? During the "Contamination" portion of the exercise,why was the contamination not present when you first placed thephenol red glucose tube in the 37 degree incubator? After yoursample had been incubated for one day, why is the solution turbid?What did the color change of the medium indicate? What caused thecontamination of your sample? How successful were you with theaseptic technique? How many times did you need to repeat theexperiment until you were able to successfully inoculate your mediawithout contamination? How important is sterile technique in a"wetlab?" Why? Conclusion < Within a few sentences, provide aconcluding statement about the results of your laboratory>

BACTERIAL GROWTHand CONTROLLINGBACTERIAL GROWTH lab report using virtual unknownsoftware

Experiment II:Controlling Microbial GrowthIntroduction

<Include purpose of lab experiment>

<brief summary of topic investigating and case studies>

<state major finding>

Procedure

< Include information that the reader would need to repeatyour experimental procedure. Do not include any observations orresults in this section>

Observations andResults

<Gram Stain Result>

Discussion &Conclusion

Include the answers to the following questions:

  • Salt is one of the oldest food preservatives. How does saltcontrol microbial growth and reduce spoilage? Which two mediainclude elevated concentrations of NaCl? Would bacteria that havethe ability to grow in these two media be more likely to cause foodspoilage? Why or why not?

  • Bile salts are natural products of the digestive system thatprevent growth of many bacteria. How do bile salts exertantimicrobial activity? What is the concentration of bile salts inbile esculin agar? Why might some bacteria be able to grow on thismedium?

  • Nitrate is often used as a preservative in cured meats toprevent anaerobic growth of spoilage bacteria. Why would theinclusion of nitrate inhibit anaerobic growth?

  • Potassium cyanide (KCN) is a potent poison. How might somebacteria be able to grow on media containing potassium cyanide?

    Conclusion

    < Within a few sentences, provide a concluding statementabout the results of both your laboratory>

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