What are Alu insertions and VNTRs. Why these polymorphisms canbe used for DNA fingerprinting? Can someone explain in a simple waywithout copy and pasting? Thanks.

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Related questions

Given the fact that DNA polymorphisms exist, clearly explain how they can be used for the cure or prevention of genetic diseases suffered by humans. In what way could restriction fragment length polymorphisms be of use in human genetic disease diagnosis, cure or prevention.Considering your response to the above question, explain how a disease you are interested in could be diagnosed, treated and possibly prevented from occurring in the future.

purplecockroach480

1. What is the size of the fragment that contains the Alu insertion? Without the Alu insertion?

2. Describe the two functions of loading dye.

3. What is the purpose of using a marker or ladder during electrophoresis?

4. Suppose you have 4 fragments of 500bp each. How many bands would appear on the gel?

5. Describe the quick stain protocol that you will be following to visualize the DNA.

6. Suppose you run a gel, and you are concerned that you may have âlostâ some of the smallest fragments due to them running off the gel. What two things can change in ensure that these would remain on the gel the next time you repeat the experiment?

1. Consider two parents. One parent does not have the insertionin either of version of her chromosome 16. The other parent doeshave the insert in both versions of his chromosome 16.

Describe DNA at this loci from their first offspring:
After PCR, samples are run through an electrophoresis gel. Wouldall offspring have identical banding patterns on an electrophoresisgel? Why or why not?

2. In Part A of the lab, you heated the samples containing yourcheek cells. Think carefully about where this DNA was packaged inyour cells, and what high temperatures usually do to enzymestructure and function. (Hint: Lysosomes contain enzymes known asDNAses!) What functions do you think these high temperatureincubations served in your DNA extractions?

3. In Part B, you added a âPCR Master Mixâ to your DNA to beginthe PCR. What ingredients did this PCR master mix have to containto allow the successful amplification of your DNA? What do each ofthese ingredients do in your reaction?

Modified (2010) from North Seattle Community College MurkowskiBiology Lab and BioRad Lab Guidelines to accompany PV-92 Kit:#166-2100EDU

4. We are using the PV-92 Alu-insert (a single locus) to obtainpopulation-genetics information about our class.

â¢ Are there other types of Alu insert, or is PV-92 the onlyone?