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27 May 2020
A scientist is worried that the protein sample she is working on has misfolded and aggregated. The results of analysis by SDS-PAGE (A), size exclusion chromatography (B), circular dichroism (C) and 1d-NMR (D) are shown below. The void volume of the size exclusion column was 8 ml. What information can you gather from each of these results below? (8 marks) What advice can you give to the scientist? (2 marks)
A scientist is worried that the protein sample she is working on has misfolded and aggregated. The results of analysis by SDS-PAGE (A), size exclusion chromatography (B), circular dichroism (C) and 1d-NMR (D) are shown below. The void volume of the size exclusion column was 8 ml. What information can you gather from each of these results below? (8 marks) What advice can you give to the scientist? (2 marks)
24 Dec 2022