Using outside resources, describe one common application of isolating plasmid DNA for molecular biological research. Note that you may NOT use the example in this week's supplementary reading (human growth hormone)

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Irving HeathcoteLv2

2 Jun 2018

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Background:

Biotechnology allows the use of living organisms or their processesfor human needs or purposes. Currently, this topic includes suchgeneral examples as cloning, stem cells (adult, umbilical cord, andembryonic), DNA fingerprinting, biological warfare, bioremediation,genetically modified organisms, vaccines, and transgenic plants andanimals to name a few. In this assignment, you will explorespecific examples of biotechnology applications.

Assignment details:

Research how a SPECIFIC biotechnology application within the aboveGENERAL examples (or ones not listed above) is used today. Example:Insulin production in bacteria is an example under the generaltopic of recombinant organisms.

What specific biotechnology application did you choose?
Briefly describe this biotechnology application. How is itdone?
Based upon the definition of biotechnology in the assignment,describe how your biotechnology application is an example ofbiotechnology.
What benefits are derived from this application?
Are there any concerns or negative impacts associated with this useof biotechnology? Why or why not?

champagnetrout780

After completing this week's reading assignment (particularly chapter 32), select a human-caused environmental issue (for example, climate change, species extinction, etc) and research it further. Find a reliable, useful website that discusses the human impacts to the environment caused by this issue . Reflect on and share the website with the class by writing a full paragraph summary (150 words minimum) of what you have learned or have found particularly useful when reviewing the website. Do not copy and paste directly from the website. This blog should be written in your own words to share your point of view. Also, include a link to the website (type the title, highlight it, then, click on the link icon to add the URL) so that your peers may also view it and benefit from the website you have discovered.

erinhare736

You believe that the cytosolic protein p007 is involved in mediating growth hormone-stimulated proliferation in cells. You wish to establish a cell model using your cultured fibroblasts. Provide clear explanations to the following, each worth 5 pts each.

a) Describe how you would transfect the growth hormone receptor DNA into your cells to create stable cell lines, and include a description of the benefits of using stable cell lines versus transiently transfected cell line.

b) Describe how you would measure specific receptor binding of GH to your stable cell line.

c) Describe how you would measure growth hormone-induced proliferation in your stable cell line. Make sure to include controls.

d) Describe how you would use RNAi to demonstrate the requirement for p007 in growth-hormone stimulated proliferation. Make sure to include controls.