Assignment details for part one:
Transcribe and translate EACH of the three following DNA genesequences. Turn these in using the graphic organizer. The lettersrepresent the DNA N-base sequences of the genes.
The first is the original gene. The next two are mutations of theoriginal. Note that the changes (mutations) are shown in red.
Original Gene sequence 3'-T A C C C T T T A G T A G C C A CT-5
Mutated gene sequence 1 3â-T A C G C T T T A G T A G C C A TT-5'
Mutated gene sequence 2 3â-T A A C C T T T A C T A G G C A CT-5â
Also, answer the following questions:
â¢What is the significance of the first and last codons of an mRNAtranscript?
â¢What meaning do these mRNA codons have for proteinsynthesis?
â¢Did the mutations result in a change in the final proteins? If so,describe the change.
â¢In general, why might a change in amino acid sequence affectprotein function?
Assignment details for part one:
Transcribe and translate EACH of the three following DNA genesequences. Turn these in using the graphic organizer. The lettersrepresent the DNA N-base sequences of the genes.
The first is the original gene. The next two are mutations of theoriginal. Note that the changes (mutations) are shown in red.
Original Gene sequence 3'-T A C C C T T T A G T A G C C A CT-5
Mutated gene sequence 1 3â-T A C G C T T T A G T A G C C A TT-5'
Mutated gene sequence 2 3â-T A A C C T T T A C T A G G C A CT-5â
Also, answer the following questions:
â¢What is the significance of the first and last codons of an mRNAtranscript?
â¢What meaning do these mRNA codons have for proteinsynthesis?
â¢Did the mutations result in a change in the final proteins? If so,describe the change.
â¢In general, why might a change in amino acid sequence affectprotein function?
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Related questions
5). The table shows five different mutations of a small section of a gene.
Use the base-pairing rules to complete the second column. For each mutation, write the sequence for any mRNA codons that will be changed as a result of the mutation in bold. Write any codons that wonât be changed in regular font.
Using the genetic code chart from question #3, identify any stop codons in the mRNA molecules â write these codons in red
For each mutation, write any amino acids that will be changed in the last column in bold font. Use red XXXs to indicate any amino acids that will be missing as a result of stop codons and use use regular font indicate any amino acids that will not be changed.
DNA (Template Strand) | mRNA codon | Polypeptide |
Original DNA = GCAAGTACCTGA | CGU UCA UGG ACU | arg â ser â trp â thr |
Mutation = GCCAGTACCTGA (nucleotide change underlined) | ____ ____ ____ ____ | ___ ___ ___ ___ |
Mutation = GCACGTACCTGA (nucleotide change underlined) | ____ ____ ____ ____ | ___ ___ ___ ___ |
Mutation = GCAAGTACTTGA (nucleotide change underlined) | ____ ____ ____ ____ | ___ ___ ___ ___ |
Mutation = GAAGTACCTGA (first C deleted) | ____ ____ ____ ____ | ___ ___ ___ ___ |
Mutation = GCAAGTACTGA (second C deleted) | ____ ____ ____ ____ | ___ ___ ___ ___ |