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28 Jul 2019

Immunology Question

1. What is TAP and what is its function? What happens to individuals who have a deficiency in TAP? What is this condition called? What are some of its associated symptoms?

2. Define the following and give an example for each: (8 marks) a) cytokine b)interleukin c) chemokine d) chemoattractant

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29 Jul 2019

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Considering the cultures to be used for inoculating each medium in this exercise, how many microbial types should you expect to see in/on each medium?

Select one:

a. 3

b. 0

c. 1

d. Several

e. 2

Which of the following is common to minimize airborne contamination?

Select one:

a. Make someone else do the experiment

b. Perform the experiment in a windy room

c. Keep a microscope nearby

d. Breathing in sample

e. Hold open tubes at an angle

How can a hot sterile flame loop be cooled quickly to use in the next streak?

Select one:

a. Put in sterile portion of agar plate

b. Blow on it

c. Stick the loop in ice

d. Set the loop on the lab bench

e. Pour tap water on the loop

True or false: Most colonies can been seen without aid (naked eye), and most single cells cannot be seen without a microscope.

Select one:

True

False

An isolated, single colony forms after incubation of a:

Select one:

a. Virus (viral particle arrangement in some cases)

b. Single cell (cell arrangement in some cases)

c. Sterile agar plate

d. Sterile flame loop

e. Liquid mixed culture

These are commonly used for growing stock cultures that can be refrigerated for several weeks:

Select one:

a. Broth tubes

b. Cotton swabs

c. Gym socks

d. Agar slants

e. Petri dishes

Beginning at the base of the slant surface and gently moving the loop back and forth as you withdraw is called:

Select one:

a. Slant mixing

b. Fishtail inoculation

c. Zig-zag streaking

d. Squirrel inoculation

e. Quadrant streaking

If you are going to mix a broth culture prior to transfer, what is a safe way to do this without special equipment?

Select one:

a. Tap the tube with your fingers

b. Vortex it

c. Use a inoculating loop

d. Stick the tube in the outer cone

What is the primary purpose of the streak plate method?

Select one:

a. Quantify CFUs

b. Isolate an individual colony

c. Quantify the cell count of a mixed culture

d. Inoculate a culture on a plate

e. Confirm the identity of an unknown genus

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