please answer all the question answering only one question is not helpful .
Practice Calculations on Serial Dilutionsâbe sure to include the amount of water added and include all units
17. Calculate and describe (or diagram) how you would use a 10% SDS solution to prepare a final volume of 20 ml of each of the following solutions: 8%, 4%, 2%, 1% 0.5%.
18. Calculate and describe (or diagram) how you would use a 5 M Tris solution to prepare 200 ml of each of the following solutions: 1M, 100 mM ,10 mM , 1 mM, 0.1 mM.
How much of the first dilution should you make to perform the following dilutions?
19. 300 ml of a 1:5 dilution
20. 100 ml of a 1:10 dilution
21. What is the minimum volume you should pipette with the following? (include units with your answers):
P2 P10 P20 P100 P200 P1000
22. How accurate was your pipetting?
23. Was it better with the larger volume or smaller volume pipettor? Provide rationale.
(If there was a consistent problem with one pipettor please tell the instructor.)
Standard Curves
24. Complete a standard curve for the data above and tape a printed version into your notebook.
25. What is the R-squared value of your trend line? Is this good or bad⦠i.e. would you repeat the assay?
26. What is the protein concentration of (Explain/show your calculations.)
Unknown #1 = Unknown #2 =
Data for DNA Concentration Procedure
Attach a labeled printout of the plate reader raw data as well as your generated standard curve to this lab.
26. What is the concentration of your unknown DNA sample?
27. What is the R 2 for your standard curve data? Why is it important that the R2 is >0.95?
28. Comment on the purity of your DNA... discuss- How can you determine the purity of DNA? What are common contaminants of DNA purified from cells?
please answer all the question answering only one question is not helpful .
Practice Calculations on Serial Dilutionsâbe sure to include the amount of water added and include all units
17. Calculate and describe (or diagram) how you would use a 10% SDS solution to prepare a final volume of 20 ml of each of the following solutions: 8%, 4%, 2%, 1% 0.5%.
18. Calculate and describe (or diagram) how you would use a 5 M Tris solution to prepare 200 ml of each of the following solutions: 1M, 100 mM ,10 mM , 1 mM, 0.1 mM.
How much of the first dilution should you make to perform the following dilutions?
19. 300 ml of a 1:5 dilution
20. 100 ml of a 1:10 dilution
21. What is the minimum volume you should pipette with the following? (include units with your answers):
P2 P10 P20 P100 P200 P1000
22. How accurate was your pipetting?
23. Was it better with the larger volume or smaller volume pipettor? Provide rationale.
(If there was a consistent problem with one pipettor please tell the instructor.)
Standard Curves
24. Complete a standard curve for the data above and tape a printed version into your notebook.
25. What is the R-squared value of your trend line? Is this good or bad⦠i.e. would you repeat the assay?
26. What is the protein concentration of (Explain/show your calculations.)
Unknown #1 = Unknown #2 =
Data for DNA Concentration Procedure
Attach a labeled printout of the plate reader raw data as well as your generated standard curve to this lab.
26. What is the concentration of your unknown DNA sample?
27. What is the R 2 for your standard curve data? Why is it important that the R2 is >0.95?
28. Comment on the purity of your DNA... discuss- How can you determine the purity of DNA? What are common contaminants of DNA purified from cells?
