In experimental conditions, the budding of clathrin-coated vesicles from fragments of the trans-Golgi network can be observed when adaptins, clathrin, dynamin, and GTP are added to the membrane preparation. What would you observe if you use all of these components except that you substitute GTP with the non-hydrolyzable GTP analog, GTPgammaS?
1)Adaptin would not bind to clathrin properly given that adaptin requires GDP in order to bind to clathrin
2)The clathrin triskelion would fall apart due to the inactivation of dynamin
3)There would be no budding from the membrane; each non-budded vesicle would remain associated with the membrane by an elongated âneckâ and polymerized dynamin 4)There would be excessive budding due to the over-activation of clathrin
In experimental conditions, the budding of clathrin-coated vesicles from fragments of the trans-Golgi network can be observed when adaptins, clathrin, dynamin, and GTP are added to the membrane preparation. What would you observe if you use all of these components except that you substitute GTP with the non-hydrolyzable GTP analog, GTPgammaS?
1)Adaptin would not bind to clathrin properly given that adaptin requires GDP in order to bind to clathrin
2)The clathrin triskelion would fall apart due to the inactivation of dynamin
3)There would be no budding from the membrane; each non-budded vesicle would remain associated with the membrane by an elongated âneckâ and polymerized dynamin 4)There would be excessive budding due to the over-activation of clathrin
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