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I am working on a project and I need to discuss how Cytochrome C. Oxidase, iron-sulfur clusters, and mRNA Splicing Factors are all related. I also need to know some interesting research topics about these 3 things together.

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Trinidad TremblayLv2

30 May 2019

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QUESTION 1

Which of the following statements regarding splicing is FALSE?

A) The length of introns determines the efficiency of splicing.

B) Many human neurological disorders are caused by splicing errors and/or mutations in splicing factors.

C) Splicing requires the action of a variety of snRNAs that direct the transesterification reactions.

D) Splicing is dictated by sequence features in pre-mRNA transcripts

QUESTION 2

Which of the following mRNA processing factors does NOT associate with the CTD of RNA polymerase II?

A) splicing factors

B) polyadenylation factors

C) miRNAs/siRNAs

D) capping proteins

QUESTION 3

Which of the following statements about mRNA stability is FALSE?

A) Deadenylation-independent mRNA decay is exclusively in the 5' to 3' direction.

B) The most common pathway of mRNA decay is deadenylation-dependent.

C) Yeast mRNA decay occurs primarily in the 5' to 3' direction, while 3' to 5' decay is more common in mammalian cells.

D) Decapping enzymes are required for all pathways of mRNA decay.

QUESTION 4

You obtain the sequence of a gene containing 10 exons, 9 introns, and a 3â² UTR containing a polyadenylation consensus sequence. The fifth exon also contains a polyadenylation site. To test whether both polyadenylation sites are used, you isolate mRNA and find a longer transcript from muscle tissue and a shorter transcript from all other tissues. Which of the following is the most likely mechanism underlying these alternative transcripts?

A) The fifth exon is spliced out of the mature mRNA transcript in muscle cells.

B) Muscle cells have less effective polyadenylation than other tissues.

C) An alternative CPSF protein is expressed in muscle cells with lower affinity for the polyadenylation sequence in exon 5.

D) The mRNA in muscle cells is edited to eliminate the polyadenylation sequence in exon 5.

blushdonkey474

As a person who is passionate about birth and aspires to be a birth worker such as a doula or midwife, I have watched a movie called, "Microbirth," which is about how the way a baby is delivered and fed after birth affects their microbiome. There are differences in the microbiome of a formula fed baby vs a breastfed baby, c-section vs delivery through the birth canal. I won't go into more details but I highly recommend it. I wonder how long these things in the early life of humans affect their future.

This article was very interesting. I've believed the gut affects the immune health, digestion of course, and skin but I didn't know about the brain-gut connection. That the microbes of the intestines would affect the mental health and mood of a person makes a lot of sense. I wonder about the ways we can have a healthy microbiome and correct an unhealthy one. Probiotics seem like they may be helpful but I'm unsure because of the lack of research. Overuse of antibacterial products may be harmful which is the opposite of what most believe.

Is this true and why?

Need help figuring out some of the structures from these pictures, I managed to get some of the answers down but I'm not too sure if they're correct since we weren't given any answer keys...(that's why there's so much, sorry!)

Also the pictures are dissections of dead mice and hearts by the way, just incase anyone can't handle the pictures.

http://imgur.com/a/O7zo4

Thank you so much!

Edit: Are there any other ways besides uploading pics onto here? Because there's about 64 images and the sizes are pretty big so I'd have to change it manually for all pictures.

Edit 2: I have the answers in all of the descriptions of the ones I do have. But here's the ones I don't know: 13,14,23,24,27,28,29,30,38,40,44-46,48

Ones I'm unsure of but I do have the answers: 4, 7, 19, 22, 33, 34, 37, 57

Edit 3: I'm not sure what you mean by incorporated. The pictures are in the link but I dont know how to post them on here without adding so many pictures.

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I am working on a project and I need to discuss how Cytochrome C. Oxidase, iron-sulfur clusters, and mRNA Splicing Factors are all related. I also need to know some interesting research topics about these 3 things together.

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