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7 Sep 2018

In eukaryotes, the first step in the control of gene expression is ______.

A. RNA processing
B. transcription
C. chromatin remodeling
D. mRNA stability control

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Irving Heathcote
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8 Sep 2018

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• Identify the mechanisms for gene expression control in the lac and trp operons

• Explain what transcription factors and regulatory proteins are and how they regulated gene expression in eukaryotes.

• Describe how chromatin structure (DNA methylation and histone modification) can affect gene expression

• Explain the process of protein degradation and its purpose

• Contrast the mechanisms regulating gene expression depending on when they act on: 1) regulating transcription, 2) posttranscriptionally (regulating the mRNA and protein synthesis) and posttranslationally (regulation of protein degradation)

he specific control of gene transcription in eukaryotes requires the complex regulation of 1) transcription factors, 2) histone modifying enzymes, and 3) chromatin remodeling factors. In your own words, describe the function of each of these three classes of transcriptional regulators (it may be helpful to use examples from the text) and how each of the components is involved in transcriptional activation and/or repression.

Can anyone elaborate on this

thank you

Eukaryotic transcription normally takes place on a chromatin template at nuclease-accessible sites.(True/False)

*After promoter clearance by RNA polymerase II:

A) all associated factors remain bound to the polymerase for elongation.

B) a subset of factors remain at the promoter region.

*Which of the following statements is FALSE?

A) RNA polymerase II is responsible for the transcription of genes encoding protein-coding sequences only.

B) Many promoters recognized by RNA polymerase II contain regulatory elements several kbp upstream of the initiation site.

C) TFIIB has a role in converting a closed-promoter into an open-promoter complex.

D) The C-terminal domain of the largest subunit of RNA pol II undergoes extensive phosphorylation prior to transcription elongation.

E) A multiprotein complex is involved in communication between upstream control elements and proteins bound at the promoter.

C) no further elongation factors are acquired by the polymerase.

D) no further chromatin remodeling is required.

E) transcription continues until the polymerase reaches the poly (A) tail

*In eukaryotes RNA polymerase I transcribes all of the ribosomal RNA components.(TRUE/FALSE)

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