If the 5â² splice site sequence changed from 5â²-GUAAGU-3â² to 5â²-GGAAGU-3â², predict the effect of the sequence change on U1 binding and U6 snRNP binding in an in vitro protein-RNA binding assay.
SUBJECT: Cell Molecular Biology
If the 5â² splice site sequence changed from 5â²-GUAAGU-3â² to 5â²-GGAAGU-3â², predict the effect of the sequence change on U1 binding and U6 snRNP binding in an in vitro protein-RNA binding assay.
SUBJECT: Cell Molecular Biology
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Part B
Think about the DNA coding sequence of a gene. If an A were swapped for a T, what kind of mutation could it cause and why?
| a) It could cause a frameshift nonsense or frameshift missense mutation because it would change the reading frame of the codon triplet. | ||||||||||||||||||||||||||||
| b) It could cause a silent, missense, or nonsense mutation because those are the types that can be caused by a nucleotide-pair substitution like this one. | ||||||||||||||||||||||||||||
| c) It could cause a nonsense mutation because the sequence would no longer be the same, so the protein would be shorter and non-functional. | ||||||||||||||||||||||||||||
| d) It could cause a silent mutation because A and T are complementary to each other so it is not really a substitution mutation. Part C Why is a frameshift missense mutation more likely to have a severe effect on phenotype than a nucleotide-pair substitution missense mutation in the same protein?
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