Question

1. Use the table below to answer the following questions:

a. Which of these is (are) “complex”? Justify your answer.

b. S. pneumoniae can grow in Medium 1 and Medium 3, but not in Medium 2. Why?

c. Would E. coli be able to grow in media 2? Justify your answer.

d. A mutation in what gene would prevent S. pneumoniae from growing in medium 3. Justify your answer. Why would this mutation have no effect on the ability of S. pneumoniae to grow in medium 1?

Medium 1	Medium 2	Medium 3
Glucose 20 g	Sodium Citrate 50 g	Lactose 20 g
Peptone 5.0g	Ammonium phosphate 1.0 g	Ammonium phosphate 1.0 g
Beef extract 3.0 g	Sodium chloride 5.0 g	Sodium chloride 5.0 g
Sheep blood 10 g	Magnesium sulfate 0.2 g	Magnesium sulfate 0.2 g
Sodium Chloride 8.0 g	Potassium phosphate 1.0g	Potassium phosphate 1.0g
Agar 15.0 g	All 20 amino acids (200 µg each)	All 20 amino acids (200 µg each)
Water 1 1iter	Guanine, adenine, thymine, cytosine, uracil (100 µg each)	Guanine, adenine, thymine, cytosine, uracil (100 µg each)
	All known vitamins (25 µg each)	All known vitamins (25 µg each)
	Water 1 liter	Water 1 liter