In lab, our objective was to obtain a mitochondrial enriched extract from cauliflower, use the mitochondrial enzyme succinate dehydrogenase as a marker for mitochondrial enrichment, and provide a better understanding of the activity of enzymes using succinate dehydrogenase as an example.
Part 1 was: Enrichment of mitochondria from cauliflower extract by differential sedimentation
Part 2 was: Monitoring of fractions for specific activity of the mitochondrial enzyme succinate dehydrogenase.
What is the general differential centrifugation scheme you perform in the differential centrifugation and SDH Activity lab?
A scientist recently identified a nuclear protein involved in nuclear lamina assembly. Which fraction of your differential centrifugation scheme would be the most enriched fraction for your protein of interest? Why?
How did you detect the enrichment of mitochondria in the isolated fractions?
Which of the fractions you collected should contain the highest enrichment of mitochondria? Does your specific activity of your fractions support this?
In order to calculate the specific activity of the isolated fractions, the protein concentration has to be determined. Why is the protein concentration necessary for accurate comparison of the enzyme activity of the fractions?
In lab, our objective was to obtain a mitochondrial enriched extract from cauliflower, use the mitochondrial enzyme succinate dehydrogenase as a marker for mitochondrial enrichment, and provide a better understanding of the activity of enzymes using succinate dehydrogenase as an example.
Part 1 was: Enrichment of mitochondria from cauliflower extract by differential sedimentation
Part 2 was: Monitoring of fractions for specific activity of the mitochondrial enzyme succinate dehydrogenase.
What is the general differential centrifugation scheme you perform in the differential centrifugation and SDH Activity lab?
A scientist recently identified a nuclear protein involved in nuclear lamina assembly. Which fraction of your differential centrifugation scheme would be the most enriched fraction for your protein of interest? Why?
How did you detect the enrichment of mitochondria in the isolated fractions?
Which of the fractions you collected should contain the highest enrichment of mitochondria? Does your specific activity of your fractions support this?
In order to calculate the specific activity of the isolated fractions, the protein concentration has to be determined. Why is the protein concentration necessary for accurate comparison of the enzyme activity of the fractions?