Question

A. Please identify the mRNA and polypeptide sequence for the below DNA using the genetic codon table. (Note: the direction of mRNA is important! Keep in mind that the genetic codons are read from 5’ to 3’ direction on mRNA. Also, RNA bases are A, U, C, G instead of A, T, C, G)

3’ – GTT – TAC – CTT – CTC – TCC – TCA – GAC – ATC – GCA – 5’ (Template strand)

5’ – CAA – ATG – GAA –GAG – AGG – AGT –CTG – TAG – CGT – 3’ (Non-template/Coding strand)

B. Please identify the mutation type (silent, missense, nonsense, frameshift) on the following DNA sequence as compared to the original sequence from question A.

3’ – GTT – TAC – CTT – CTC – TTC – TCA – GAC – ATC – GCA – 5’ (Template strand) 5’ – CAA – ATG – GAA –GAG – AAG –AGT – CTG – TAG – CGT – 3’(Non-template/Coding strand)

C. Please identify the mutation type (silent, missense, nonsense, frameshift) on the following DNA sequence as compared to the original sequence from question A.

3’ – GTT – TAC – CTT – ATC – TCC – TCA – GAC – ATC – GCA – 5’ (Template strand)

5’ – CAA – ATG – GAA –TAG – AGG – AGT – CTG – TAG – CGT – 3’(Non-template/Coding strand)