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10 Jan 2019
1) Describe important steps needed to express the gene coding for protein x if protein x were isolated from eukaryotic organism.
2) You are intrested in detecting the expression of the gene coding for protein X in R.palustris in the presence and absance of specific antibiotic.Name and breifly describe the technique that you would use. Justfy your reasoning.
3) you are intrested in silencing expression of wild-type gene that codes for protein x without disrupting or modifying the actual gene and its regulatory regions. What assay would you use and why?
1) Describe important steps needed to express the gene coding for protein x if protein x were isolated from eukaryotic organism.
2) You are intrested in detecting the expression of the gene coding for protein X in R.palustris in the presence and absance of specific antibiotic.Name and breifly describe the technique that you would use. Justfy your reasoning.
3) you are intrested in silencing expression of wild-type gene that codes for protein x without disrupting or modifying the actual gene and its regulatory regions. What assay would you use and why?
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Jamar FerryLv2
11 Jan 2019