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17 Apr 2018
1. You used a standard curve to estimate the molecular weight of your unknown protein(s), based on the KD value(s) measured. Considering how this was accomplished, why is there likely to have been an exceptionally large error associated with this estimate?
2. You are given a solution containing a mixture of three colorless proteins and various contaminating salts. How could you use size exclusion chromatography to isolate and purify these proteins (both separating them and ridding them of the salts), and how could you locate or track where the target proteins eluted?
1. You used a standard curve to estimate the molecular weight of your unknown protein(s), based on the KD value(s) measured. Considering how this was accomplished, why is there likely to have been an exceptionally large error associated with this estimate?
2. You are given a solution containing a mixture of three colorless proteins and various contaminating salts. How could you use size exclusion chromatography to isolate and purify these proteins (both separating them and ridding them of the salts), and how could you locate or track where the target proteins eluted?
1
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Jamar FerryLv2
20 Apr 2018