Explain how the Sanger Method works and discuss the importance of sequencing in biomedicine

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Tod ThielLv2

2 Jul 2018

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Related questions

. Next Generation DNA Sequencing methods have dominated the study of microbial diversity in the last 12 years. Compare and contrast the Sanger vs the Illumina approach to DNA sequencing. First explain how each approach works, first the Sanger method then Illumina. Then, compare those two methods based on various Explain the principles of NGS its advantages and disadvantages as a tool for the characterization of microbial diversity.

DNA Sequencing (Sanger method):

https://www.dnalc.org/resources/animations/sangerseq.html

Questions:

5. In what ways are PCR and Sanger sequencing similar?

6. What is the important difference in Sanger sequencing?

7. Why is size separation of DNA fragments so important in Sanger sequencing? How are the DNA fragments separated in the animation?

Additional videos on Sanger DNA sequencing:

Sanger Sequencing

https://www.youtube.com/watch?v=6ldtdWjDwes

The Sanger Method of DNA Sequencing

https://www.youtube.com/watch?v=FvHRio1yyhQ

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