What is occurring in the cells as they leave the maximum exponential phase and approaching stationary phase? What could be added or subtracted from the culture that would return the population to its maximum exponential rate?

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Hubert KochLv2

3 Feb 2018

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amethystsquirrel762

A student working with E. coli wants to run an experiment on a population that is as dense as possible without actually going into stationary phase. In LuriaBertani broth, commonly used to grow E. coli, cells begin entering stationary phase at a cell density of 5 Ã 107 cells/mL. She inoculates a 10 mL culture with 1000 cells and sets it up in a shaking incubator, knowing that after about an hour of lag time E. coli should double every 20 minutes. How long can she go play Hearthstone before she has to get back for her experiment?