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11 Nov 2019
Make a 120 ml agarose gel of 0.7% (w/v) that is 0.5X TBE. The TBE (Tris, Borate, EDTA) stock is 5X and FW agarose is 210. You have a 50X stock of buffer and you need 1 50 mu 1 of 1x. How do you make this? You have a DNA solution that is 125 mu 1: how much ethanol do you add if you add 2X the volume of ethanol? Make 500 ml of a 10X TE stocks (Tris, EDTA). 1X TE is 10mM Tris and ImM EDTA. FW of Tris is 121.1 and EDTA is 187.
Make a 120 ml agarose gel of 0.7% (w/v) that is 0.5X TBE. The TBE (Tris, Borate, EDTA) stock is 5X and FW agarose is 210. You have a 50X stock of buffer and you need 1 50 mu 1 of 1x. How do you make this? You have a DNA solution that is 125 mu 1: how much ethanol do you add if you add 2X the volume of ethanol? Make 500 ml of a 10X TE stocks (Tris, EDTA). 1X TE is 10mM Tris and ImM EDTA. FW of Tris is 121.1 and EDTA is 187.
Elin HesselLv2
1 Mar 2019