EI Noncompetitive inhibition is a limiting case in which the effect of binding inhibitor has no effect on the affinity for the substrate and vice versa. Suggest what a Lineweaver-Burk plot would look like for an inhibitor that had a reaction scheme similar to standard noncompetitive inhibition (shown at right), but where binding inhibitor lowered the affinity of El for the substrate ES ESI The slope of the plot in the presence of the inhibitor will be greater thanthe slope in the absence of the inhibitor. The 1/V axis intercept of the plot in the presence of the inhibitor will be higher than concentrations will intersec the intercept in the absence of inhibitor. Plots for separate experiments at different inhibitor on the 1/[S] axis on the 1/W axis where 1/S] = 0 and 1/ 0 where 1/[S > 0 and 1/W>0 where 1/[S] 0 where 1/[S]