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11 Nov 2019
1. If the half-time of a first order reaction is 0.35, what is the rate constant k? 2. At what substrate concentration will an enzyme having a maximum substrate- transformation velocity of 30umoles/min x mg and a Km of 0.005M show 1/4 of its maximum rate? 3. Calculate Km and Vmax from the following data (using all 3 forms of linear graphs). 0.1 0.2 0.4 0.8 1.6 0.34 0.53 0.74 0.91 1.04 4. Using the definations of Vmax and Km, estimate (do not graph) the approximate value of Vmax and Km for the enzyme-catalyzed reaction from which the following data were obtained. . EwW ISIM 2.5 x 10-6 4.0 x 10-6 1.0 x 10-5 2.0 x 10-5 4.0 x 10-5 1.0 x 10-4 v (umol/Lx min) 28 40 70 95 112 128 5. What is the order of the reaction below? v = k[A][B]2 What is the order of the reaction with respect to A? What is the order of the reaction with respect to B?
1. If the half-time of a first order reaction is 0.35, what is the rate constant k? 2. At what substrate concentration will an enzyme having a maximum substrate- transformation velocity of 30umoles/min x mg and a Km of 0.005M show 1/4 of its maximum rate? 3. Calculate Km and Vmax from the following data (using all 3 forms of linear graphs). 0.1 0.2 0.4 0.8 1.6 0.34 0.53 0.74 0.91 1.04 4. Using the definations of Vmax and Km, estimate (do not graph) the approximate value of Vmax and Km for the enzyme-catalyzed reaction from which the following data were obtained. . EwW ISIM 2.5 x 10-6 4.0 x 10-6 1.0 x 10-5 2.0 x 10-5 4.0 x 10-5 1.0 x 10-4 v (umol/Lx min) 28 40 70 95 112 128 5. What is the order of the reaction below? v = k[A][B]2 What is the order of the reaction with respect to A? What is the order of the reaction with respect to B?
Keith LeannonLv2
30 Apr 2019