Question

Although graphical methods are employed for accurate determination of the V_max and K_m of an enzyme-catalyzed reaction, sometimes these quantities can be quickly estimated by inspecting values of the initial rates, v, at increasing [S]. Estimate the V_max and K_m values of the enzyme-catalyzed reaction for the following data shown in the columns below:

[S], M v, (?moles/min/?g of protein) = initial rate 2.5 x 10-6 28 4.0 x 10-6 40 1 x10-5 70 2 x 10-5 95 4 x 10-5 112 1 x 10 -4 128 2 x 10-3 139 1 x 10-2 140