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11 Nov 2019
1. PLP mechanism! (20 points). In class we discussed the diversity of enzyme-catalyzed reactions, especially involving amino acid substrates, that can be mediated by the cofactor PLP. Shown below is a PLP-mediated reaction of serine that is an important step in the conversion of this amino acid to other carbon building blocks in the cell. The active site lysine sidechain plays a starring role in this mechanism. Not only does it form a Schiff base with PLP in the enzyme active roles as well. site, as shown below, but plays additional Substrate (serine) Products Active site Lysm Active site OH O H20 NH2 NH2 PLP cofactor a. Draw a complete mechanism (using appropriate arrow pushing) for this enzyme- catalyzed reaction. b. Which of the catalytic strategies we discussed in class are used by this enzyme? For each strategy you identify, explain how it is used by this enzyme (1-2 sentences per strategy)
1. PLP mechanism! (20 points). In class we discussed the diversity of enzyme-catalyzed reactions, especially involving amino acid substrates, that can be mediated by the cofactor PLP. Shown below is a PLP-mediated reaction of serine that is an important step in the conversion of this amino acid to other carbon building blocks in the cell. The active site lysine sidechain plays a starring role in this mechanism. Not only does it form a Schiff base with PLP in the enzyme active roles as well. site, as shown below, but plays additional Substrate (serine) Products Active site Lysm Active site OH O H20 NH2 NH2 PLP cofactor a. Draw a complete mechanism (using appropriate arrow pushing) for this enzyme- catalyzed reaction. b. Which of the catalytic strategies we discussed in class are used by this enzyme? For each strategy you identify, explain how it is used by this enzyme (1-2 sentences per strategy)
Irving HeathcoteLv2
17 Jan 2019