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You have a mixture of Glycine, Lysine, and Glutamic acid that you want to separate by carbon exchange chromatography. After binding to the column, you apply an increasing pH gradient to elute them (going from pH 0 to 14 for example). What is the order of elution of the amino acids (first, second, last? Lys, Gly, Glu Gly, Glu, Lys Glu, Gly, Lys Gly, Lys, Glu
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Related questions
A protein of interest needs to be separated from a mixture of proteins for amino acid sequencing. It is known that the protein of interest is highly enriched in lysine residues compared to the other proteins in the mixture. Which of the following techniques will be most effective in isolating this protein?
options:
a) | gel-filtration chromatography | ||||||||||
b) | SDS-polyacrylamide gel electrophoresis | ||||||||||
c) | mass spectrometry | ||||||||||
d) | cation exchange chromatography | ||||||||||
e) | anion exchange chromatography 2. Consider a mixture of three peptides. The three peptides have the following primary structure: A) asp-asp-val B) glu-asp-glu C) val-val-lys Determine the net charge of each peptide at pH 7. From this, in what order will these peptides elute from an anion exchange column at pH 7? options:
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