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17 Nov 2019

Part D Suggest a method for separating the peptides produced by chymotrypsin treatment. Check all that apply.

(1) Chromatography on a cationic column, at pH=7

(2) Chromatography on a anionic column, at pH=4

(3) Size exclusion chromatography

(4) Electrophoresis

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Related questions

A protein of interest needs to be separated from a mixture of proteins for amino acid sequencing. It is known that the protein of interest is highly enriched in lysine residues compared to the other proteins in the mixture. Which of the following techniques will be most effective in isolating this protein?

options:

a)

gel-filtration chromatography
b)

SDS-polyacrylamide gel electrophoresis
c)

mass spectrometry
d)

cation exchange chromatography
e)

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2. Consider a mixture of three peptides. The three peptides have the following primary structure:

A) asp-asp-val

B) glu-asp-glu

C) val-val-lys

Determine the net charge of each peptide at pH 7. From this, in what order will these peptides elute from an anion exchange column at pH 7?

options:

a)

A will elute first, followed by B then C.
b)

B will elute first, followed by C then A.
c)

C will elute first, followed by A then B.
d)

B will elute first, followed by A then C.
e)

Not enough information is provided to determine the elution order.

Sapling Learning Help!

Chromatography is a technique in which a gas or liquid mobile phase carries the analytes (compounds to be separated) through a stationary phase and then to a detector that measures the analytes one-by-one as they exit the chromatographic system. Match the numerical terms in the first list with the appropriate alphabetical definitions in the second list. Note: choose as many matches as applicable.

1. adsorption chromatography

2. ion-exchange chromatography

3. size exclusion chromatography

4. capillary electrophoresis

Ions in mobile phase are attracted to counterions covalently attached to the stationary

phase.

Solute equilibrates between mobile phase and film of liquid attached to stationary

phase.

Solute in mobile phase is attracted to specific groups covalently attached to the

stationary phase.

D. Solute equilibrates between mobile phase and surface of stationary phase.

E. Different-sized solutes penetrate voids in stationary phase to different extents.

Largest solutes are eluted first.

F. Charged ions are separated based on their electrophoretic mobility with the use of an

applied voltage.

G. Migration of compounds occurs due to their different degrees of interaction with the

stationary phase.

H. Utilizes a very strong mobile phase (k=0).

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6. predict the expected product of acetylation of 4-bromobiphenyl.

7. predict the structure of the major diacetylated product expected from biphenyl.

8. why does benzoylation not occur twice on the same ring of ferrocene?

9. account for the fact that ferrocene is eluted by the nonpolar solvent, petroleum ether, during column chromatography

10. what feature of the benzoylation of ferrocene makes it particularly attractive for a column chromatography experiment?

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