What is the mechanism of gel filtration chromatography? What are the advantages and disadvantages of gel filtration chromatography? What other protein purification techniques could be used to purify albumin or any protein from a complex mixture of proteins? Assuming that your protein of interest is an enzyme, how would you evaluate the purification techniques?
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Related questions
A protein of interest needs to be separated from a mixture of proteins for amino acid sequencing. It is known that the protein of interest is highly enriched in lysine residues compared to the other proteins in the mixture. Which of the following techniques will be most effective in isolating this protein?
options:
a) | gel-filtration chromatography | ||||||||||
b) | SDS-polyacrylamide gel electrophoresis | ||||||||||
c) | mass spectrometry | ||||||||||
d) | cation exchange chromatography | ||||||||||
e) | anion exchange chromatography 2. Consider a mixture of three peptides. The three peptides have the following primary structure: A) asp-asp-val B) glu-asp-glu C) val-val-lys Determine the net charge of each peptide at pH 7. From this, in what order will these peptides elute from an anion exchange column at pH 7? options:
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