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11 Dec 2019

Using the Henderson-Hasselbach equation

1. Calculate the charge of Cysteine side chain at pH 1.5 and pH 10.5. (pKa of Cysteine side chain is 8.4)

2. Calculate the charge of Histidine side chain at pH 1.5 and pH 10.5. (pKa of Cysteine side chain is 6)

PLEASE SHOW THE CALCULATION ON HOW THE CHARGE WAS OBTAINED. THANKS!!

This is a biochemistry question

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Reid Wolff
Reid WolffLv2
13 Dec 2019

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Related questions


Amino acid side chain charge calculation (approximation)
1. Write out ionic forms of the amino acids and determine acid and base charge forms. 2. Use the Henderson-Hasselbach equation to calculate ratio
3. Assigncharge(approximation):

If 10%, assign plus/minus 0.9 or 0.10 values. If 99% assign whole number (or 1% of whole and 99% of whole). If at pKa assign half value 0.5 (plus/minus)

a) The amino acid sequence of sea lamprey adrenocorticotropic hormone is given below. Given the sequence, identify each ionizing amino acid residue and indicate its charge (-1, 0, or +1) in the completely protonated amino acid form of the polypeptide hormone.

S-V-S-S-P-K-Y-A-M-G-H-F-R-W-G-S-P-D-K-A-T-I

b)

Given the same sequence above, indicate the charge (-1, 0, or +1) on each amino acid residue in

the completely proton dissociated form of the polypeptide hormone.

Below is protien sequence showsn using single letter abbreviations from an insect mid gut. The pH of the midgut for this insect is pH 10.5 and that is the optimal pH for this protien.

1) Above each amino acid side group label the charge [+,-,N (neutral) ] for each amino acid side group, along this protien at its optimal pH.

2) Then, calculate the overall cahrge "net charge" for this protien when present in the insect midgut.

N-terminus: M-A-R-K-E-K-F-W-P-Y-H-R-C-Y-S-D-R-G

~So I believe I am looking for the charges at a pH of 10.5, but not sure?

I know KRH (+) and DE(-) have charges in neutral pH

Optimal temps K: 10.0 R:12.0 H:6.5 D:4.4 E:4.4 Is it correct then that the charges would be

N-N-N-+-N+-N-N-N-N-+-N-N-N-N-N-N-N???

M-A-R-K-E-K-F-W-P-Y-H-R-C-Y-S-D-R-G

I also don't understand how to do number two or how to factor in the amnio and carboxyl groups with pH C:3.1 and N:8.0??

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