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13 Dec 2019

C1V1=C2V2
I have to make 6 SMX solutions that have concentration range from 3.0E-5 M to 1.0E-4 M

C1= (1.0E-4) * (10ml) / (7.5ml) = 1.33E-4M

Find V1 for other 6 solutions and its concentration, what total V of C1 solution is needed, what vol of C1 should you make, what mass of SMX do you need?

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Please help; I am seriously confusing myself. My data is below followed by question(s) that are stumping me. The Given dilution formula (C₁V₁ = C₂V₂) in the question does not match what is in my lab handout which is (A₁A_{2 =} C₁C₂) is it possible one is wrong. I do not necessarily need all three questions answered - please show me how to set up one and I should be able to set up & deduce the others!

Experiment 2: Measure Absorbance versus Concentration for Each Dye

Placed four clean cuvettes on the workbench.

Filled each cuvette with the amounts 0.020 mM Yellow 5 dye stock solution & water as shown in the table below.

Dye Solution Water % Dye

cuvette 1 10.0mL none 100%

cuvette 2 7.5mL 2.5mL 75%

cuvette 3 5.0mL 5.0mL 50%

cuvette 4 2.5mL 7.5mL 25%

Yellow 5 dye

Cuvette #1

Wavelength = 440 absorbance = 0.48

Cuvette #2

Wavelength = 440 absorbance = 0.36

Cuvette #3

Wavelength = 440 absorbance = 0.24

Cuvette #4

Wavelength = 440 absorbance = 0.12

What was the concentration of the food dyes in cuvette 1? You can use the dilution formula below.

C₁V₁ = C₂V₂

A. 0.20 mM

B. 0.010mM

C. 0.020 mM

D. 0.0050 mM

What was the concentration of the food dyes in cuvette 2? You can use the dilution formula below.

C₁V₁ = C₂V₂

A. 0.0050 mM

B. 0.015 mM

C. 0.020 mM

D. 0.20 mM

What was the concentration of the food dyes in cuvette 3? You can use the dilution formula below.

C₁V₁ = C₂V₂

A.0.20 mM

B.0.010 mM

C.0.020 mM

D.0.0050 mM

1. 62.0

m L

of 8.5

(m / v) %

K C l

(MW = 74.55

g / m o l

)

Express your answer using two significant figures.

2.250.0

m L

of 9.0

(m / v) %

acetic acid (MW = 60.05

g / m o l

)

Express your answer using two significant figures.

3.If you had only 25

g

K O H

remaining in a bottle, how many milliliters of 6.5

% (w / v)

olution could you prepare?

Express your answer using two significant figures.

4.How many milliliters of 0.17

M

solution could you prepare?

Express your answer using two significant figures.

5.Calculate the mass percent (m/m) of a solution prepared by dissolving 46.79

g

N a C l

in 160.5

g

H_{2} O

Express your answer to four significant figures.

6.Calculate the mass percent (m/m) of a solution prepared by dissolving 46.79

g

N a C l

in 160.5

g

H_{2} O

Express your answer to four significant figures.

7.Calculate the mass/volume percent (m/v) of 14.0

g

N a C l

in 61.5

m L

of solution.

Express your answer to three significant figures.

8.Calculate the molarity (

M

) of 153.5

g

H_{2} S O_{4}

in 1.085

L

of solution.

Express your answer to four significant figures.

9.What is the boiling point of a solution made using 827

g

of sucrose,

C_{12} H_{22} O_{11}

, in 0.200 k

k g

of water,

H_{2} O

10.What is the freezing point of a solution that contains 29.5

g

of urea,

C O (N H_{2})_{2}

, in 205

m L

water,

H_{2} O

? Assume a density of water of 1.00

g / m L

11.Given the formula

C_{1} V_{1} = C_{2} V_{2}

, where

C C

indicates concentration and

V V

indicates volume, which equation represents the correct way to find the concentration of the dilute solution (

C_{2} C_2

12.What is the final volume

V_{2} V_2

in milliliters when 0.935

L

of a 48.0

%

(m/v) solution is diluted to 24.7

%

(m/v)?

13.A 738

m L

N a C l

solution is diluted to a volume of 1.15

L

and a concentration of 3.00

M

. What was the initial concentration

C_{1} C_1

Express your answer with the appropriate units.

tealdog287

Hello, I am doing homework and I am not sure if my answers are correct, can someone help me verify if I am doing this right or wrong? My responses are underneath the questions.

You are given a protein solution with a concentration of 0.25 mg/ml. Answer these questions.

A.) We need 20 Âµg for an experiment. What volume of the protein solution do we need?

Convert 20 Î¼g and you will get 0.02 mg C = M/V rearrange for Volume V= M/C , V= (0.02 mg)/(0.25 mg/ ml) = 0.08 ml or 80 Î¼l

B.) Suppose we want to prepare a solution containing 150 Âµg of the protein at a concentration of 0.50 mg/ml. To do this we will first dry down enough protein solution to get 150 Âµg. How much solution do we need to start with? How much H2O do we add to get the desired concentration?

You will convert 150 Î¼g to 0.15 mg and divide by 0.25 mg/ ml to get 0.60 ml that needs to be dried down to obtain 0.5 mg/ml, then you will add enough water so that the total volume is 0.5 ml.

C.) If the protein has a M.W. = 15,000, express its initial concentration in moles/liter, in Âµmoles/ml and in Âµmoles/Âµl. If we want 2 Âµmoles for a reaction, what volume do we need?

Convert 0.25 mg/mL = 0.00025 g/mL = 0.00025 g/0.001 L = 0.25 g/L If M.W. of the protein is 15,000 g/mol then 0.25 g = 0.25/15,000 moles = 1.67 x 10^-5 moles. This many moles is contained in a liter (L) therefore concentration is 1.67 x 10^-5 moles/L = 1.67Î¼moles/L = 1.67 Î¼moles/1000 mL = 1.67 x 10^-3 Î¼moles/mL = 1.67 x 10^-3 Î¼moles/1000 Î¼L = 1.67 x 10^-6 Î¼moles/Î¼L. The concentration of the solution is 1.67 x 10^-6 Î¼moles/Î¼L, 1 Î¼mole is present in 1/(1.67 x 10^-6) Î¼L and if 2 Î¼moles are present in 2/(1.67 x 10^-5) Î¼L = 1.20 x 10^-5 Î¼L = 1.20 L

D.) Suppose we want 1 ml of a 10 Âµg/ml solution. How much H2O and protein stock must we add to get this?

C1V1 = C2V2 (250 Î¼g/ Î¼l)(V1) = (10 Âµg/ml)(1ml) V1= 0.04 ml or 40 Î¼l (Volume of protein stock for solution) Volume of water 1.0 ml- 0.04ml = 0.96 ml

E.) Suppose we want 100 Âµl of a 0.1 Âµg/Âµl solution. How much H2O and protein stock must we add to get this?

C1V1 = C2V2 (0.25 Î¼g/ Î¼l)(V1)= (0.1 Î¼g/ Î¼l)(100 Î¼l) V1=40.0 Î¼l (Volume of protein stock for solution) Volume of water 100 Î¼l- 40.0 Î¼l = 60.0 Î¼l

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C1V1=C2V2
I have to make 6 SMX solutions that have concentration range from 3.0E-5 M to 1.0E-4 M

C1= (1.0E-4) * (10ml) / (7.5ml) = 1.33E-4M

Find V1 for other 6 solutions and its concentration, what total V of C1 solution is needed, what vol of C1 should you make, what mass of SMX do you need?

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C1V1=C2V2 I have to make 6 SMX solutions that have concentration range from 3.0E-5 M to 1.0E-4 M C1= (1.0E-4) * (10ml) / (7.5ml) = 1.33E-4M Find V1 for other 6 solutions and its concentration, what total V of C1 solution is needed, what vol of C1 should you make, what mass of SMX do you need?

Related textbook solutions

Basic Chemistry

Principles of Chemistry Molecular Approach

Chemistry: Structure and Properties

Principles of Chemistry Molecular Approach

Chemistry: A Molecular Approach

Chemistry: A Molecular Approach

Principles of Chemistry: A Molecular Approach

Chemistry: The Central Science

Related questions

Weekly leaderboard

C1V1=C2V2
I have to make 6 SMX solutions that have concentration range from 3.0E-5 M to 1.0E-4 M

C1= (1.0E-4) * (10ml) / (7.5ml) = 1.33E-4M

Find V1 for other 6 solutions and its concentration, what total V of C1 solution is needed, what vol of C1 should you make, what mass of SMX do you need?