Question

a) Calculate how you would make 1.0 L of 10x TBE (0.9 M Tris, 0.9 M Boric Acid, 20 mM EDTA) Tris MW=121.4 g/mole; Boric Acid MW=61.84 g/mole; EDTA MW=292.2 g/mole

b) For your polyacrylamide gel electrophoresis gel you need 50 mL of 0.5x TBE. Calculate and describe how you would make this solution using your stock solution.