1. Transfer RNA exists in two forms that are in equilibrium A<--> B , with equilibrium constant K = [B]eq/[A]eq = 10.0 at40 ^oC. A 10 microM solution of tRNA is very rapidlyheated from 37 ^oC to 40 ^oC and a relaxationtime for reaching the new equilibrium of t = 3 ms is measured.

a) Find the rate constants for the forward and reverse reactionsfor tRNA isomerization. What temperature do these values correspondto?

b) If we double the tRNA concentration, will the rate constantsand relaxation time change or stay the same?

Question

1. Transfer RNA exists in two forms that are in equilibrium A<--> B , with equilibrium constant K = [B]eq/[A]eq = 10.0 at40 ^oC. A 10 microM solution of tRNA is very rapidlyheated from 37 ^oC to 40 ^oC and a relaxationtime for reaching the new equilibrium of t = 3 ms is measured.

a) Find the rate constants for the forward and reverse reactionsfor tRNA isomerization. What temperature do these values correspondto?

b) If we double the tRNA concentration, will the rate constantsand relaxation time change or stay the same?

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