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18 Dec 2019

A.
If you add 1ml of 1M HCL to 100ml of 0.045M Tris Base. What will be the resulting ph? (Pka of tris = 8.3

B.how many grams of tris base and how many mL of 1m HCL would you need to make 700mL of a 0.8M Tris buffer at ph 7.6. The pjs of Tris is 8.3.

C.
How many grams of Na2HPO4 (141.96g/Mol) and NaH2PO4(119.96g/mol) are needed to make 700ml of 0.4m phosphate buffer at ph 6.3? The pka of phosphate is 7.21

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Hubert Koch
Hubert KochLv2
31 Dec 2019

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Related questions

Buffer Calculations Practice (how much to mix?)

PART A:

Make a 300 ml solution of 100mM Tris base in H2O. CALCUALTE

Use the henderson hasselbach to make a 50 ml 50mM Tris solution at pH 8.0 by mixing Tris HCl and Tris base powders ( use formula weights of Tris-HCl and Tris base). CALCULATE How many grams of Tris HCl and Tris base did you add to make your solution?

PART B: HOW DO YOU MAKE THE FOLLOWING BUFFER; CALCULATE

Buffer: 10X TAE

0.4M Tris base

0.01M disodium EDTA

0.2M acetic acid

Make 500ml of this solution from chemical stocks. The pH of this solution should be between 8.2 and 8.3.

THEN Make a 100 ml 1X dilution of TAE from this 10X stock. CALCULATE

A)how many grams of tris (121 g/mol) and tris-h (122 g/mol) and tris-H (122 g/mol) are needed to make 500 mL of a M tris buffer at ph 8.2. the pka of tris is 8.3.

B)what molarity of Naoh is needed so that 22 mL diluted to 83 mL will yield 13 M. include the appropriate unit.

C)how many milliliters of 9.1 M HNO3 are required to prepare 100 mL OF 0.2 HNO3??

Tris buffer [a shortened form of Trizma, tris(hydroxymethyl)aminomethane] is very common buffer for biochemical applications. One buys Trizma*HCl, (C4H11NO3*HCl, the acid) and the Trizma base (C4H11NO3, the base) and mixes them in appropriate amounts to make a buffer of the desired pH. If you desire 500 mL of a pH 7.2 solution, how many grams of Trizma*HCl and Trizma base should be dissolved in 500 mL of water? Make the buffer 0.050 M total Tris concentration. The pKa of Triz is 8.1 at 25 degrees Celsius

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