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1. What is natural selection and what is artificial selection?What evidence for natural selection can we gain by studyingartificial selection?
2. List the sequences of the mRNA molecules transcribed from thefollowing DNA template sequences Also, translate the associatedamino acid sequence for each.
T G A - T A G - T T G - C T T - G A G - A G T
m RNA =
A A =
3. Using the following template, replicate DNA.
Template: 3' A A G - G T C - T G A - T G G - C C A - T A G5'
Complementary strand:
1. The Pribnow Box is a
A. attenuator
B. enhancer
C. promoter
D. operator 2. Which of the following do not occur during post-transcriptional processing of RNA in eukaryotes?
A. the splicing of introns
B. addition of a poly-A at the 3' end
C. removal of introns
D. addition of a 7-methyl guanosine cap at the 5' end 3. In transcription, RNA polymerase binds at
A. a promoter
B. an operator
C. the oriC locus
D. an enhancer 4. The Central Dogma in genetics describes
A. the pattern of information flow in the cell
B. the pattern of chromosomal inheritance in populations
C. the role of mutations in disease
D. the role of promoters 5. Which of the following sequences of DNA could code for a stem-loop structure (only the template strand is shown)?
A. 3'--G-G-C-C-C-T-T-G-G-A-A-A-A-C-C-A-A-G-G-G-C-C--5'
B. 3'--G-G-C-C-C-T-T-G-G-A-A-A-A-G-G-T-T-C-C-C-G-G--5'
C. 3'--G-G-C-C-C-T-T-G-G-T-T-T-T-G-G-C-C-C-T-T-G-G--5'
D. none of the above 6. Removal of introns from RNA is by
A. primosomes
B. peptidases
C. galactosidases
D. spliceosomes 7. Which of the following represents the units for measuring the rate of sedimentation of a molecule/organelle in a sucrose gradient?
A. molecular weight
B. Svedberg units
C. map units
D. Daltons 8. During transcription, which strand is not read by the RNA polymerase?
A. coding strand
B. noncoding strand
C. template strand
D. terminator strand 9. The function of the rho protein is
A. to help terminate translation
B. to help RNA polymerase bind to the DNA
C. to help RNA polymerase find a promoter
D. to help terminate transcription 10. The unusual bases found in transfer RNAs
A. are added during transcription
B. are normal bases that have been modified after transcription
C. help the tRNAs to bind to DNA
D. ensure that the tRNA stays linear in solution
1. What was the purpose of using blender to liquefy the onion before adding the DNA lysis. 2. What was the purpose of heating the onion solution before filtering it it and adding the ethanol? 3. what was the purpose of adding ethanol to the filtered DNA solution. 4. what is the template DNA for the following RNA strand. AUGCCGAUCCGUUAAG 5. If the RNA in the previous strand were translated, what would the order of the amino acid chain be? 6. List one possible mRNA strand that would code for the following amino acid chain: Mathionine, proline, glycine, glutamic acid, alanine. Don't forget to add the stop codon. 7.List the template DNA that would correspond to the mRNA strand in the previous question. 8. What amino chain would the following template DNA strand code for:--T A C G G C C C C G G A T T A A T T-? show your work?