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11 Dec 2019

What makes bacterial plasmids ideal vectors for foreign DNA? Name at least one other vector that is based on a virus?

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12 Dec 2019
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Related questions

Based on what you now know about bacterial transformation, predict the impact of inserting a very large foreign DNA fragment (5+ Kb) into pUC18 on….

a. the number of plasmids replicated in each cell

b. the efficiency of transformation (i.e., the percentage of cells that took up the plasmid)

c. the phenotype (color) of the colonies transformed with the plasmid.

Most temperate phages integrate into the host chromosome, whereas some replicate as plasmids. Which kind of relationship do you think would be more likely to maintain the phage in the host cell, and why?

A.Plasmids-they're smaller, so they'd be easier to replicate by the host cell.

B.Integrated-the host cell would be less likely to view this DNA as "foreign" on subsequent rounds of replication, and would retain it more easily.

C.Integration-because plasmids are frequently lost during cell division, which could leave a daughter cell without the virus genome.

D.Plasmids-these structures often carry other genes that may give the host cell a selective advantage over cells that don't have them.

As a scientist involved in gene therapy, you have been asked to develop gene therapy vectors based on one of these viruses to treat Parkinson’s disease and other diseases of the nervous system.

Which virus would you use as a gene therapy vector? Clearly explain why you picked this virus based on what you know about the replication cycle of the virus.

How would you design this vector? Think about where in the genome of the virus would you insert the transgene to be delivered to the nervous system and why?​

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