MCDB 423 Lecture Notes - Lecture 7: Mitosis, Subventricular Zone, Wild Type

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Golgi staining only labels a small number of cells. Cajal discovered that the brain is made out of cells and individual cells have unique structures and shapes. They are organized in a very highly orchasted and complex organization and highly specified. Individual neurons take on distinct shapes and take on distinct functions and are in distinct functions in the brain. A key aspect to making these hypothesis about the brain was the ability to see individual neurons. If you just stain for cells (nuclei, ribosomal organelles, etc. ) you see that there is lots of cells. A key to understand specific organizations is to see a subset of cells. The technique cajal had was called the golgi staining technique. It"s a d(cid:455)e (cid:449)hi(cid:272)h (cid:449)ill for(cid:373) a rea(cid:272)tio(cid:374) i(cid:374) the (cid:272)ell a(cid:374)d fill the (cid:272)ell (cid:449)ith a dark (cid:272)olor (cid:271)ut o(cid:374)l(cid:455) happens stocastly (very low efficient; labels only a small amount of cells)

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