MBIO 1010 Lecture Notes - Lecture 30: Listeria Monocytogenes, Pancreatic Cancer, Chemotherapy
8 May 2017
School
Department
Course
Professor
Document Summary
Through research, frederick banting discovered mechanism of diabetes . Insulin was 1st produced in 1921 by f. banting and charles best harvested insulin from animal (dog) pancreas . Although similar to human insulin, bovine and porcine insulin are not the same . Many patients form antibodies against the foreign protein . Fear of long-term affects of using foreign protein . Stimulated search for new ways of making human insulin in large volumes . Solution: insert human insulin gene into e. coli produce human insulin recombinant dna technology . Body sees it as foreign when it comes into the body so we destroy it with an immune response. Two chains: : chain 21 a. a. Bacteria still ha(cid:448)e li(cid:373)itatio(cid:374)s si(cid:373)ple pol(cid:455)peptide is ok Synthesis of dna containing the nucleotide sequences of the a and b polypeptide chains of insulin . Dna containing the a-chain gene and b-chain gene individually cloned into a plasmid vector .
