BIO120H1 Lecture Notes - Lecture 4: Dna Supercoil, Reverse Transcriptase, Telomerase
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BIO120H1 Full Course Notes
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Lecture outline: issues in replication, dna repair. Micro-rays: in each location on micro-ray, certain dna sequence has been spotted & it sticks there. Have some yeast, then add fluorescent dntps, stimulate dna replication, isolate dna & apply it to micro-ray & it will hybridize to specific sequences on micro-ray. At 20 minutes, dna is fully replicated. After you harvest each 1 of these populations of yeast that you allowed to replicate for different amounts of time, separate strands & fluorescently label them. & then you hybridize them to what is know as micro-ray. In cases where you do not have any replication (kind of like control 0 min) see light green dots indicate unreplicated dna while replicated dna are indicated by dark green dots. As you progress, have larger patches of dna micro-ray itself is composed of yeast genome in little fragments that are arranged sequentially from left to right.
