BIOL308 Study Guide - Rna Polymerase Iii, Rna Polymerase Ii, Rna Polymerase I

• School: University of Waterloo
• Department: Biology
• Course: BIOL308
• Professor: Dragana Miskovic

Document Summary

The process of maturation also includes adding a 5" cap and a 3" poly a tail: the chromatin structure in eukaryotes limits access of the enzymes to the genes. This helps regulate the process of transcription. (0. 01% transcriped at any given moment, approximately 3 genes). N. b: rnap iv plays a role in gene silencing. Hypothesis: there are 3 rnaps in eukaryotes (i, ii and iii). It also proved they played different roles in transcription. It"s a lipid soluble molecule (can cross the membrane) found in mushrooms (death cap and destroying angel). It can be used to identify the type of rnap in action since rnap i is insensitive to it, rnp ii is highly sensitive and rnap iii is only slightly sensitive. Actinomycin d is an antibiotic produced by streptomyces that intercalates into gc rich regions of dna inhibiting their transcription. (rnap can no longer bind to them) rnap i is the most sensitive to it.