CSB349H1 Lecture Notes - Lecture 5: Deoxyribonuclease I, Nucleosome, Chromatin
Document Summary
Csb349 lecture 5 transcription i: genome structure and transcription. Slide 4 model of nucleosome remodelling in a nut shell. When the genome is in a condensed and closed state, there won"t be any transcription. Transcriptional activators bring the closed state to an open state. Class i: writing and erasing making marks on the chromatin. Class ii: reading and doing reading marks made by class i and do stuff. Slide 6 chemical modification of histones within nucleosomes. Histone tails: flexible parts of the chromatin that do not adopt a crystal structure, where the class i enzymes will make their marks (e. g. , covalent modifications) Slide 7 two classes of chromatin remodeling enzymes. There are enzymes that make the histone marks. There are also enzymes that take off the histone marks. Transcription factors, atp-dependent nucleosomal remodeling enzymes (class ii) and other histone modifying enzymes are recruited once the histone marks are made. Slide 8 identifying modifications by chromatin immunoprecipitation (chip)
