BIOL 205 Lecture Notes - Rna Polymerase Ii, Chromatin Remodeling, Tata Box
22 Nov 2013
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B/c of additional oh: much more susceptible to degradation. Opposite dna strands can serve as template for rna. Christmas tress = shpw direction transcription, thousands of polymerases jumping on gene. Many rnas can be simultaneously transcribed from a gene. Miller spread: electronic microscopic examination of rna transcription if frogs eggs. Spread out on grid and examine with electron microscopy. Ribosomal rna: upon fertilization zygotic dna can not launch protein synthesize it needs, mum deposits ribosomes in egg (utilizing thousands of rna genes she made) @-35: region that is highly conserved signal that tells specific proteins to start assembly on. Accommodate large rna polymerase so always starts at tata box. Sigma interacts w/ dna binding unit and catalytic unit to bring it to the right spot. 80% of all e. coli dna contain this hairpin loop. Creates a stem loop (not gc rich) In prokaryotic cells: transcription can occur before translation has even finished.
